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사이토 카인 캡처 시스템을 사용하여 임상 응용 프로그램에 대한 거대 세포 바이러스 고유의 자동화 된 세포 농축은 T 세포
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
DOI:

10:24 min

October 05, 2015

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Capitoli

  • 00:05Titolo
  • 02:00Automated Cell Enrichment
  • 05:03Cell Count Determination
  • 06:35Examination of the Separation Performance
  • 07:54Results: Enrichment of CMV-specific T Cells Using the CCS System
  • 09:29Conclusion

Summary

Traduzione automatica

The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.

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