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Neuronale Differenzierung von embryonalen Stammzellen der Maus in Serum-freiem Monolayer-Kultur
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
DOI:

06:09 min

May 14, 2015

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Capitoli

  • 00:05Titolo
  • 01:10Plating the Cells
  • 02:56Immunofluorescent Staining
  • 04:13Results: Representative Neural Differentiation from Mouse Embryonic Stem Cell Cultures
  • 05:45Conclusion

Summary

Traduzione automatica

This protocol describes in detail the method for generating neural progenitors from embryonic stem cells using a serum-free monolayer method. These progenitors can be used to derive mature neural cell types or to study the process of neural specification and is amenable to multiwell format scaling for compound screening.

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