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水生微生物Metaproteomics工作流程:从细胞对胰蛋白酶肽适合串联质谱为基础的分析
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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
DOI:

08:09 min

September 15, 2015

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Capitoli

  • 00:05Titolo
  • 01:06Perform Cell Lysis in Catridge Filter Unit with Cells Preserved in RNA Stabilization Solution
  • 04:07Protein Precipitation and Peptide Extraction
  • 05:25DNA Precipitation
  • 06:49Results: Taxonomic and Functional Analysis of Arctic Ocean Microbial Communities
  • 07:50Conclusion

Summary

Traduzione automatica

This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.

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