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באתרו איתור של חיידקים בתוך רקמות מוטבעות-פרפין באמצעות DNA בדיקה שכותרת Digoxin מיקוד 16S rRNA
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Capitoli
- 00:05Titolo
- 01:35Making the Probe
- 04:02In Situ Hybridization
- 06:53Applying an Antibody and Counter Stain
- 08:56Results: Comparing Eubacterial and P. gingivalis-specific Probes and Using the Eubacterial Probe for General Bacterial Detection Purposes
- 10:32Conclusion
Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.
Tags
In Situ DetectionBacteriaDigoxin-labeled DNA Probe16S RRNAPeriodontitisGingival BiopsiesElectron MicroscopyImmunohistochemistryImmunofluorescenceFluorescent In Situ Hybridization (FISH)Species-specificUniversalBacterial FormsEpitheliaInflammatory InfiltratesBlood VesselsPeriodontitisCancerInflammatory Immune Diseases
