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Capitoli
- 00:05Titolo
- 01:30Pulse Labeling of Cells with BrdU
- 03:41Cell Fixation and Permeabilization
- 04:37Antibody Staining and DNA Staining
- 06:12Results: Flow Cell Analysis
- 08:28Conclusion
This protocol describes the use of bromodeoxyuridine (BrdU) uptake to permit the temporal tracking of cells that were in S phase at a specific point in time. Addition of DNA dyes and antibody labeling facilitates detailed analysis of the fate of the S phase cells at later times.
