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Isolering och förökning av cirkulerande tumörceller från en mus Cancer
JoVE Journal
Medicina
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JoVE Journal Medicina
Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model
DOI:

05:22 min

October 09, 2015

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Capitoli

  • 00:05Titolo
  • 01:10Collection of Whole Blood for Circulating Tumor Cell Isolation
  • 02:08Lysis of RBC in the Buffy Coat
  • 03:19Propagation of Circulating Tumor Cells
  • 04:29Results: Isolation of Circulating Tumor Cells
  • 04:56Conclusion

Summary

Traduzione automatica

Circulating tumor cells (CTCs) have been shown to play an important role in tumor metastasis. Here, a method for the isolation and propagation of CTCs from the whole blood of a syngeneic mouse tumor model of hepatocellular carcinoma (HCC) metastasis is described.

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