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Capitoli
- 00:05Titolo
- 01:28Calcium-phosphate Transfection of HEK293T Cells
- 03:05Preparation of Cell Lysates for Co-immunoprecipitation
- 04:09Preparation of Immune Complexes and Protein G Beads
- 05:38Immunoprecipitation of the Immune Complexes by Protein G Beads and their Elution
- 08:00Results: Mx1 Interacts with Influenza A Virus Nucleoprotein
- 09:03Conclusion
This co-immunoprecipitation protocol allows to study the interaction between the influenza A virus nucleoprotein and the antiviral Mx1 protein in human cells. The protocol emphasizes the importance of N-ethylmaleimide for successful co-immunoprecipitation of Mx1 and influenza A virus nucleoprotein.
