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Einrichtung von einem klinisch relevanten<em> Ex Vivo</em> Mock Kataraktchirurgie Modell zur Untersuchung von epithelialen Wundheilung in einer nativen Mikromilieu
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment
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Einrichtung von einem klinisch relevanten<em> Ex Vivo</em> Mock Kataraktchirurgie Modell zur Untersuchung von epithelialen Wundheilung in einer nativen Mikromilieu

DOI:
10.3791/52886-v

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07:53 min

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June 05, 2015

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, , ,
1Pathology, Anatomy and Cell Biology,Thomas Jefferson University

Capitoli

  • 00:05Titolo
  • 01:20Setup and Preparation of Lenses for Ex Vivo Wound Culture
  • 03:31Performing Mock Cataract Surgery
  • 04:43Preparing the Wounded Lens for Ex Vivo Culture
  • 05:35Separation of the Central Migration and Original Attachment Zone
  • 06:17Results: Injury Response of Cells in their Native Microenvironment
  • 07:18Conclusion

Summary

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Described here is the establishment of a clinically relevant ex vivo mock cataract surgery model that can be used to investigate mechanisms of the injury response of epithelial tissues within their native microenvironment.

Tags

Ex VivoCataract SurgeryEpithelial Wound RepairLens EpitheliumLens CapsuleLens Fiber CellsVimentinMesenchymal ProgenitorsWound Healing ResponseNative Microenvironment

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