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Capitoli
- 00:05Titolo
- 02:05Cell Culture and DNA Damage Induction
- 03:13TEM Sample Preparation
- 08:10TEM Imaging
- 09:30Image Processing
- 10:50Results: Electron Spectroscopic Imaging Describes Foci Changes Following DNA Damage
- 12:21Conclusion
Electron spectroscopic imaging can image and distinguish nucleic acid from protein at nanometer resolution. It can be combined with the miniSOG system, which is able to specifically label tagged proteins in transmission electron microscopy samples. We illustrate the use of these technologies using double-strand break repair foci as an example.
