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Direkte Induktion af Hemogenic Endothelium og Blood af Overekspression af transkriptionsfaktorer i Human pluripotente stamceller
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Direkte Induktion af Hemogenic Endothelium og Blood af Overekspression af transkriptionsfaktorer i Human pluripotente stamceller

DOI:
10.3791/52910-v

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08:14 min

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December 03, 2015

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, ,
1Primate Research Center,University of Wisconsin-Madison, 2Department of Pathology and Laboratory Medicine,University of Wisconsin School of Medicine and Public Health

Capitoli

  • 00:05Titolo
  • 00:58Induction of Hematoendothelial Precursors from Human Pluripotent Stem Cells
  • 03:29Induction of Hematoendothelial Precursors from hPSCs (Day 1-7)
  • 05:29Results: Differentiation of Blood Cells from Endothelial Cells
  • 06:35Conclusion

Summary

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This protocol describes the efficient induction of hemogenic endothelium and multipotential hematopoietic progenitors from human pluripotent stem cells via the forced expression of transcription factors.

Tags

Hemogenic EndotheliumHematopoietic CellsPluripotent Stem CellsTranscription FactorsETV2GATA1GATA2TAL1LMO2Endothelial-hematopoietic TransitionErythroidMegakaryocyticIn Vitro Modeling

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