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Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders
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Neuroscienze
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JoVE Journal Neuroscienze
Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders

DOI:
10.3791/52919-v

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07:43 min

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May 12, 2015

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, , , ,
1Centre for Integrative Biology (CIBIO),University of Trento, 2CNR Neuroscience Institute, Pisa, Italy

Capitoli

  • 00:05Titolo
  • 01:30Preparation of Brain Sections
  • 02:16pERK Immunohistochemistry
  • 05:22Bright-field Imaging of Neuron Activation
  • 05:43Results: pERK is a Reliable Molecular Readout of Learning Dependent Neuronal Activation
  • 06:45Conclusion

Summary

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We describe an immunohistochemistry protocol to study the profile of hippocampal neuron activation following exposure to a spatial learning task in a mouse model characterized by cognitive deficits of neurodevelopmental origin. This protocol can be applied to both genetic or pharmacological mouse models characterized by cognitive deficits.

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Keywords: ImmunohistochemistryPERKHippocampusSpatial LearningMorris Water MazeEngrailed-2 KnockoutAutism Spectrum DisordersNeurodevelopmental DisordersNeuronal Activation

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