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Fluoreszenz Biomembrane Kraft Sonde: Concurrent Quantifizierung von Rezeptor-Ligand-Kinetik und Binding-induzierte intrazelluläre Signaling auf Einzelzell
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1Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences,Georgia Institute of Technology, 2Coulter Department of Biomedical Engineering,Georgia Institute of Technology, 3Charles Perkins Centre,The University of Sydney, 4Institute of Biophysics, Laboratory of RNA Biology,Chinese Academy of Sciences, 5University of Chinese Academy of Sciences, 6School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Zhejiang University
Capitoli
- 00:05Titolo
- 02:28Human RBCs Isolation, Biotinylation and Osmolarity Adjustment
- 04:07Preparation for Micropipettes and a Cell Chamber
- 06:24BFP Experiment
- 10:25Fluorescence BFP (FBFP) Experiment
- 11:51Results: Representative T-cell Receptor and Peptide MHC Binding Kinetics Data & Calcium Fluorescence Data from the FBFP Experiment
- 13:14Conclusion
