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Capitoli
- 00:05Titolo
- 01:30Isolation of Primary Mouse Hepatocytes
- 05:27Fatty Acid Oxidation Assay
- 08:55Lipogenesis Assay
- 10:13Results: Fatty Acid Oxidation and Lipogenic Responses to Respiration Altering Compounds
- 11:33Conclusion
De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and lipogenesis.
