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En reference Broth mikrofortynding Metode til dalbavancin<em> In vitro</em> Resistensbestemmelse af bakterier, der vokser aerobt
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

En reference Broth mikrofortynding Metode til dalbavancin<em> In vitro</em> Resistensbestemmelse af bakterier, der vokser aerobt

DOI:
10.3791/53028-v

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11:28 min

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September 09, 2015

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, ,
1Laboratory Specialists, Inc, 2Clinical Microbiology, Actavis plc

Capitoli

  • 00:05Titolo
  • 02:00Preparation of Minimum Inhibitory Concentration (MIC) Panel Dilutions
  • 03:30Preparation of MIC Panels
  • 04:13MIC Panels, Purity Check, and Colony Count
  • 08:31Representative Dalbavancin MIC Results
  • 10:46Conclusion

Summary

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Here, we present a protocol for determination of dalbavancin susceptibility of clinically relevant Gram-positive bacteria using a broth microdilution reference methodology.

Tags

Antimicrobial Susceptibility TestingDalbavancinBroth MicrodilutionCLSIISODMSOPolysorbate 80Staphylococcus AureusMRSAStreptococcusEnterococci

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