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实施膜片钳和活荧光显微镜监视新鲜分离的PKD上皮细胞的功能特性

JoVE Journal
Biologia

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JoVE Journal Biologia

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

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实施膜片钳和活荧光显微镜监视新鲜分离的PKD上皮细胞的功能特性

DOI:

10.3791/53035-v

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08:46 min

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September 01, 2015

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Tengis S. Pavlov, Daria V. Ilatovskaya, Oleg Palygin, Vladislav Levchenko, Oleh Pochynyuk, Alexander Staruschenko

¹Department of Physiology,Medical College of Wisconsin, ²Department of Integrative Biology & Pharmacology,University of Texas Health Science Center at Houston

Capitoli

00:05Titolo
02:14Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments
05:02Single Channel Patch-clamp Electrophysiology
05:32Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells
06:55Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats
08:08Conclusion

Summary

Traduzione automatica

表现在肾小管上皮细胞离子通道在多囊肾病的病理一个显著的作用。这里，我们描述用于执行囊性上皮从啮齿动物肾脏新鲜分离的膜片钳分析和细胞内钙水平的测量的实验方案。

Tags

Keywords: Patch Clamp Live Fluorescence Microscopy Polycystic Kidney Disease PKD ARPKD Ion Channels Calcium Signaling Epithelial Monolayer Ex Vivo Analysis PCK Rat Sprague Dawley Rat Native Setting Epithelial Isolation Immunostaining Primary Culture Biochemical Assays

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