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Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism (Microcystis aeruginosa) Using Flow Cytometry
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JoVE Journal Medicina
Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism (Microcystis aeruginosa) Using Flow Cytometry

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism (Microcystis aeruginosa) Using Flow Cytometry

DOI:
10.3791/53036-v

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10:16 min

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January 29, 2016

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, ,
1Department of Life and Environmental Sciences, Faculty of Science and Technology,Bournemouth University

Capitoli

  • 00:05Titolo
  • 00:42Initial Cell Count Determination
  • 04:03Molecular Probe Cell Uptake Optimization
  • 06:02Molecular Probe Fluorescence Discrimination
  • 07:24Results: Representative Cell Mortality Analyses by Cytometry
  • 09:10Conclusion

Summary

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Microbial populations contain substantial cell heterogeneity, which can dictate overall behavior. Molecular probe analysis through flow cytometry can determine physiological states of cells, however its application varies between species. This study provides a protocol to accurately determine cell mortality within a cyanobacterium population, without underestimating or recording false positive results.

Tags

Molecular ProbeCell MortalityPhotosynthetic OrganismMicrocystis AeruginosaFlow CytometryCyanobacterial PhysiologyFluorescent ProbeCell PhysiologyMicrobial CommunitiesReal-time AnalysisCytometry LabBournemouth UniversityHemolysis TubeSample Injection ProbeFluorescenceLight ScatterBackground NoiseM. Aeruginosa MonocultureAlgal MediumCell DisaggregationLight MicroscopyForward Light ScatterNatural FluorescencePhycocyaninEmission Detection

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