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Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
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Biologia
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JoVE Journal Biologia
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

DOI:
10.3791/53059-v

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10:00 min

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October 12, 2015

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, , , , ,
1Department of Physiology,Medical College of Wisconsin

Capitoli

  • 00:05Titolo
  • 02:03Sensor Calibration
  • 04:38Animal Surgeries
  • 06:09Data Acquisition Ex Vivo
  • 07:23Results: Microelectrode Biosensor Detection of Analytes in Whole Kidneys
  • 08:57Conclusion

Summary

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Enzymatic microelectrode biosensors enable real-time measurements of extracellular cell signaling in biologically-relevant concentrations. The following protocols extend the applications of biosensors to the ex vivo and in vivo detection of ATP and H2O2 in the kidney.

Tags

Keywords: Enzymatic BiosensorsATPH2O2KidneyReal-time DetectionAmperometryGlycerol KinaseGlycerol-3-phosphate OxidasePlatinum/iridium ElectrodeGold ElectrodeCatalaseApyraseCalibrationEx VivoIn Vivo

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