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正向遗传学的方法来揭开抗逆基因小鼠 - 在ES细胞高通量筛选
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells
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正向遗传学的方法来揭开抗逆基因小鼠 - 在ES细胞高通量筛选

DOI:
10.3791/53062-v

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15:40 min

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November 11, 2015

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, ,
1Department of Cell and Developmental Biology,University of Colorado Denver

Capitoli

  • 00:05Titolo
  • 00:48Construction of a Gene-trapped Embryonic Stem (ES) Cell Library
  • 06:11Replication of the ES Cell Library
  • 09:49Stress Selection and Isolation of Resistant Clones
  • 13:14Results: Forward Genetic Screen for Stress Resistance Mutations
  • 14:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

应力性的标志长寿之一，已知是基因支配。在这里，我们开发了一个不带偏见的高通量方法筛选，赋予抗逆性ES细胞以开发小鼠模型长寿研究突变。

Tags

Genetic ScreenForward GeneticsEmbryonic Stem CellsHigh-throughputStress ResistanceParaquatOxidative StressMouse ModelsGene Function

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