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Capitoli
- 00:05Titolo
- 00:33Perfusion and Digestion of Liver Tissue Sample
- 02:36Isolation of Hepatocytes
- 03:56Isolation of Non-parenchymal Liver Cells
- 05:16Separation of Kupffer Cells, Endothelial Cells, and Stellate Cells
- 07:07Results: Morphology of Human Parenchymal and Non-parenchymal Liver Cells in Suspension and After Adherence
- 07:57Conclusion
A technique to isolate human hepatocytes and non-parenchymal liver cells from the same donor is described. The different liver cell types build the basis for functional liver models and tissue engineering. This new method aims to isolate liver cells in a high yield and viability.
