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Oberfläche Verbreitung und Immunfärbung von Hefechromosomen
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Biologia
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JoVE Journal Biologia
Surface Spreading and Immunostaining of Yeast Chromosomes
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Oberfläche Verbreitung und Immunfärbung von Hefechromosomen

DOI:
10.3791/53081-v

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12:06 min

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August 09, 2015

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, ,
1Department of Radiation and Cellular Oncology,University of Chicago, 2Department of Molecular Genetics and Cell Biology,University of Chicago

Capitoli

  • 00:05Titolo
  • 01:56Preparation of Spheroplasts
  • 03:51Chromosome Spreading
  • 06:07Immunostaining
  • 09:36Results: Optimal Chromosome Spreading with and without the Use of Lipsol
  • 11:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

A method for surface-spreading chromosomes from budding yeast is presented. This method is derived from a method previously described by Loidl and Klein. In addition, we demonstrate a procedure for immunostaining of spread chromosomes.

Tags

Keywords: Surface SpreadingImmunostainingYeast ChromosomesSaccharomyces CerevisiaeChromatin-bound ProteinsMitotic Cell CycleMeiotic ChromosomesRecombinosomesSynaptonemal ComplexDetergent TreatmentFixationLight Microscopy

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