Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

Lianguo Wang; Nicole M. De Jesus; Crystal M. Ripplinger

doi:10.3791/53166

Rivista

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Biologia

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的内肌浆网钙光学标测 2+和的Langendorff灌注兔心脏跨膜电位

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Biologia

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JoVE Journal Biologia

Optical Mapping of Intra-Sarcoplasmic Reticulum Ca²⁺ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

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的内肌浆网钙光学标测 2+和的Langendorff灌注兔心脏跨膜电位

DOI:

10.3791/53166-v

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09:26 min

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September 10, 2015

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Lianguo Wang, Nicole M. De Jesus, Crystal M. Ripplinger

¹Department of Pharmacology,University of California Davis, ²Departments of Pharmacology and Biomedical Engineering,University of California Davis

Capitoli

00:05Titolo
01:49Preparations
03:31Harvesting, Perfusing and Dye-loading a Rabbit Heart
07:09Optical Mapping
07:56Resulting V_m and SR Ca²⁺ Signals from a Langendorff-perfused Rabbit Heart
08:59Conclusion

Summary

Traduzione automatica

本文介绍了详细的协议和必要的跨膜电位_（V M）双光学测绘和免费内部肌浆网（SR）设备^钙在的Langendorff灌注兔心脏。此方法允许直接观察和第V _米量化和SR ^的 Ca 2+动力学在完整心脏。

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