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集成的无人类诱导多能干细胞的生成方法头发衍生角质形成细胞
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
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集成的无人类诱导多能干细胞的生成方法头发衍生角质形成细胞

DOI:
10.3791/53174-v

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08:36 min

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August 20, 2015

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, ,
1Centre for Eye Research Australia & Department of Ophthalmology,University of Melbourne

Capitoli

  • 00:05Titolo
  • 01:08Isolation of Keratinocytes From Plucked Hair
  • 03:16Maintenance and Passaging of Keratinocytes
  • 04:34Generation of hiPSCs from Keratinocytes using Episomal Vectors
  • 07:25Results: iPSCs Derived from Keratinocytes Express Pluripotent Markers
  • 08:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.

Tags

Keywords: Hair-derived KeratinocytesHuman Induced Pluripotent Stem CellsHiPSCsNon-invasiveReprogrammingEpisomal VectorsRegenerative MedicineDisease ModelingIn Vitro Drug ScreeningGene Therapy

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