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Die Produktion von Doppelstrang-DNA-Ministrings
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Production of Double-stranded DNA Ministrings
DOI:

06:12 min

February 29, 2016

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Capitoli

  • 00:05Titolo
  • 00:39Preparation of Media and Cultures
  • 01:58Growth Phase
  • 03:19Ministring Production and Harvesting
  • 04:31Results: Double-stranded Ministring Production
  • 05:13Conclusion

Summary

Traduzione automatica

The linear covalently closed (LCC) DNA minivector (DNA ministring) is a non-viral gene delivery vector offering high transfection efficiency and is relevant to any DNA delivery application. The production system is simple, rapid, and scalable in vivo. The following protocol provides visual step-by-step instructions for the production of DNA ministrings.

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