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采用数字液滴PCR技术检测BRAF V600E突变的福尔马林固定石蜡包埋的参考标准细胞系
JoVE Journal
Biologia
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JoVE Journal Biologia
Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
DOI:

10:16 min

October 08, 2015

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Capitoli

  • 00:05Titolo
  • 01:23DNA Extraction from FFPE Reference Standard Cell Lines
  • 03:33Digital Droplet PCR: Droplet Generation
  • 05:56Digital Droplet PCR: PCR Amplification
  • 07:36Digital Droplet PCR: Droplet Reading and DNA Mutation Profiling
  • 08:28Results: ddPCR Detects BRAF V600E Mutations in FFPE Reference Standard Cell Lines
  • 09:20Conclusion

Summary

Traduzione automatica

该视频的目标是展示如何执行从福尔马林固定的石蜡包埋(FFPE)的参考标准细胞系和数字液滴的PCR(ddPCR)分析的自动化DNA提取,以检测稀有突变在临床环境中。 FFPE样品中检测突变表明ddPCR的FFPE样品中的临床应用。

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