Rivista
/
Biologia dello sviluppo
/
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

DOI:
10.3791/53202-v

•

09:40 min

•

September 30, 2015

•

, ,
1Department of Neuroscience, Biomedical Center,Uppsala University

Capitoli

  • 00:05Titolo
  • 01:33Egg Handling and Eye Collection
  • 03:21Preparation of Whole Retinal Explants
  • 04:15Electroporation of Whole Retinal Explants
  • 07:28Results: Uses of Cultured Whole Retinal Explants
  • 08:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes a method to dissect, experimentally manipulate and culture whole retinal explants from chicken embryos. The explant cultures are useful when high success rate, efficacy and reproducibility are needed to test the effects of plasmids for electroporation and/or reagent substances, i.e., enzymatic inhibitors.

Tags

Keywords: Whole Retinal ExplantsChicken EmbryosElectroporationChemical ReagentsExperimental ModelCentral Nervous SystemIntraocular InjectionsEx Vivo CultureControlled ExposureRapidReproducibleCost-effective

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/53202?article_type=v

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
check_url/it/53202?article_type=v

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
check_url/it/53202?article_type=v

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation
check_url/it/53202?article_type=v

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
check_url/it/53202?article_type=v

In Ovo Electroporation in the Chicken Auditory Brainstem
check_url/it/53202?article_type=v

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
check_url/it/53202?article_type=v

Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
check_url/it/53202?article_type=v

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
check_url/it/53202?article_type=v

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
check_url/it/53202?article_type=v

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

Read Article