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AC-6フィーダシステムを使用してリンパ球系共通前駆細胞からマウス形質細胞様樹状細胞のインビトロ生成
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
DOI:

08:18 min

November 23, 2015

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Capitoli

  • 00:05Titolo
  • 01:03Bone Marrow Cell Isolation
  • 03:25Common Lymphoid Progenitor (CLP) Co-culture and Flow Cytometric Analysis
  • 06:01Results: Representative Murine CLP-derived pDC
  • 07:28Conclusion

Summary

Traduzione automatica

In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.

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