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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
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JoVE Journal Medicina
VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

DOI:
10.3791/53215-v

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15:07 min

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December 28, 2015

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, , , , ,
1Department of Medicine,Weill Cornell Medical College, 2Institute for Computational Biomedicine,Weill Cornell Medical College, 3Department of Pathology and Laboratory Medicine,Weill Cornell Medical College

Capitoli

  • 00:05Titolo
  • 00:41DNA Extraction from Tumor Samples
  • 04:00DNA Quality Assessment
  • 05:33VDJ PCR
  • 07:30VDJ Amplicon Library Preparation
  • 12:32Results: VDJ-sequencing Traces Clonal Evolution Patterns of B Cell Lymphoma Relapse
  • 14:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes an approach to interrogate the recombined immunoglobulin heavy chain VDJ regions of lymphomas by deep-sequencing and retrieve VDJ rearrangement and somatic hypermutation status to delineate clonal architecture of individual tumor. Comparing clonal architecture between paired diagnosis and relapse samples reveals lymphoma relapse clonal evolution modes.

Tags

VDJ-seqImmunoglobulin Heavy ChainB Cell LymphomaClonal EvolutionNext-generation SequencingSomatic HypermutationClonal HeterogeneityTumor RelapseMinimal Residual DiseaseImmune Repertoire

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