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In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling
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Capitoli
- 00:05Titolo
- 01:04Silanization and Biomolecule Functionalization of Glass Slide
- 03:25Determination of Pulsatility Index
- 04:39Flow Chamber Assembly
- 05:56Results: Cell Morphology and Protein Expression Changes with Flow Conditions
- 06:48Conclusion
This protocol replicates physiological or pathological blood flow in vitro to aid in determining cell response in disease pathologies. By introducing a pressure damping chamber downstream of a blood pump, blood flow across the vasculature can be recapitulated and imposed on a monolayer of vascular endothelium or a mimetic co-culture.
