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Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

DOI:
10.3791/53252-v

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11:37 min

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November 24, 2015

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, , , ,
1Department of Genetics,University of Cambridge, 2Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences

Capitoli

  • 00:05Titolo
  • 00:39Cell Culture and Generation of Aggregates
  • 03:12Stimulation of Cells in Aggregates
  • 04:29Cell Aggregate Fixation, Permeabilization and Blocking
  • 07:05Antibody Binding and Detection
  • 09:53Results: Morphological Evaluations of mESC Aggregate Culture
  • 10:57Conclusion

Summary

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Traduzione automatica

We have developed a protocol to generate aggregates of mouse embryonic stem cells that display self-organization, symmetry breaking and elongation paralleling axial development. This technique allows the study of axial developmental processes and the generation of cell types that are otherwise difficult to perform in monolayer culture.

Tags

Keywords: Mouse Embryonic Stem CellsEmbryoid BodySelf-organizationSymmetry BreakingAxial ElongationCell Fate SpecificationGastrulation-like MovementsImmunocytochemical AnalysisAxial DevelopmentCellular Decision-making

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