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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
 

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Article DOI: 10.3791/53265-v • 06:20 min • December 24th, 2015
December 24th, 2015 •
1, 2, 1, 3, 1
1Regenerative Medicine Research Group, Institute of Cellular & System Medicine, National Health Research Institutes (NHRI), 2National Institute of Cancer Research, National Health Research Institutes (NHRI), 3Institute of Microbiology & Immunology, National Defense Medical Center

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The immunomodulatory properties of human mesenchymal stem cells (MSC) appear increasingly relevant for clinical application. Using a co-culture system of MSCs and peripheral blood leukocytes pre-stained with the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE), we describe the in vitro assessment of MSC immunomodulation on effector leukocyte proliferation and specific subpopulations.

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Immunomodulatory Properties Human Mesenchymal Stem Cells MSCs Clinical Use Immune-related Diseases Leukocyte Proliferation Fluorescent Cellular Dye Carboxyfluorescein Succinimidyl Ester (CFSE) Flow Cytometric Analyses Cell Divisions Population Of Proliferating Cells Intracellular Cytokine/factor Expression Magnetic Bead Surface Marker Selection Peripheral Blood Mononuclear Cell Populations Co-culture Assay Cellular Interactions
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