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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

DOI:
10.3791/53296-v

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10:22 min

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November 12, 2015

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1Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas,Universidad Autónoma del Estado de Morelos, 2Instituto de Biotecnologìa,Universidad Nacional Autónoma de México

Capitoli

  • 00:05Titolo
  • 00:57Preparation of the Subnuclear Fraction Enriched in Adenoviral Replication Compartments
  • 05:54Immunofluorescence Visualization of RCf
  • 08:00Results: Presence of Adenoviral Components in the Replication Compartment Fraction from Infected Human Foreskin Fibroblasts
  • 08:49Conclusion

Summary

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We provide a novel strategy to isolate viral replication compartments (RC) from adenovirus (Ad)-infected human cells. This approach represents a cell-free system that can help to elucidate the molecular mechanisms regulating viral genome replication and expression as well as regulation of viral-host interactions established at the RC.

Tags

Keywords: AdenovirusReplication CompartmentsNuclear MicroenvironmentsViral GenomeViral ProteinsCellular ProteinsAntiviral ResponseDNA Damage ResponseInnate Immune ResponseVelocity GradientsCell-free SystemHost-cell Interactions

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