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Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

DOI:
10.3791/53314-v

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09:28 min

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November 16, 2015

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, ,
1Department of Pathology and Immunology,University of Geneva

Capitoli

  • 00:05Titolo
  • 01:00Bone Marrow Single Cell Suspension Preparation
  • 02:38Neutrophil Enrichment
  • 04:18Neutrophil Labeling
  • 05:29In vivo Measurement of Neutrophil and Monocyte Behavior
  • 07:44Results: Representative Intravital Neutrophil and Monocyte Imaging
  • 08:46Conclusion

Summary

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We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

Tags

Keywords: Intravital MicroscopyLeukocyte MigrationMonocytesNeutrophilsCX3CR1gfp/wt MousePam3SK4Vascular InflammationLeukocyte Transendothelial MigrationReal-time Imaging

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