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通过流式细胞仪测量附件和流感的内在病毒在A549细胞
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry
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通过流式细胞仪测量附件和流感的内在病毒在A549细胞

DOI:
10.3791/53372-v

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07:25 min

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November 04, 2015

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,
1Institute of Medical Virology,University of Zurich

Capitoli

  • 00:05Titolo
  • 01:20Attachment/ Internalization Assay
  • 05:25Results: Analysis of Internalization of Influenza A Virus in A549 Cells
  • 06:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present a protocol describing a semi-quantitative method for measuring both, the attachment of influenza A virus to A549 cells, as well as the internalization of virus particles into the target cells by flow cytometry.

Tags

Influenza A VirusAttachmentInternalizationA549 CellsFlow CytometryBiotinylationStreptavidinCell Permeabilization

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