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RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
JoVE Journal
Ambiente
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JoVE Journal Ambiente
RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

DOI:
10.3791/53398-v

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09:44 min

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December 21, 2015

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, ,
1National Peanut Research Laboratory,United States Department of Agriculture, Agricultural Research Service

Capitoli

  • 00:05Titolo
  • 00:41Preparing the Seeds and Controls
  • 04:40Aflatoxin Analysis
  • 06:58RT-PCR Analysis for Transgene Expression
  • 07:20RNAi-conferred Resistance to Aflatoxins
  • 09:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

We demonstrate a method for the analysis of aflatoxins and transgene expression in peanut seeds that contain RNA-interference signals for silencing aflatoxin-synthesis genes in the fungus Aspergillus flavus. RNAi-mediated control of mycotoxins in plants has not been reported previously.

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Keywords: RNAiAflatoxinsPeanutAspergillusMycotoxinReal-time PCRUPLCTransgenicFood ContaminationFood Safety

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