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Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
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JoVE Journal Medicina
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

DOI:
10.3791/53405-v

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07:42 min

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November 26, 2015

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, , , , ,
1Department of Pathology,University of Illinois at Chicago, 2Department of Periodontics,University of Illinois at Chicago

Capitoli

  • 00:05Titolo
  • 00:59Tissue Preparation
  • 02:49Toluidine Blue Staining of PEN-frame Slides
  • 03:35Laser-capture Micro-dissection (LCM)
  • 06:23Results: Tissue Collection by Laser Capture Microdissection
  • 06:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

The goal of this protocol is to use laser-capture micro-dissection as an effective method to isolate pure populations of cell types from heterogeneous prostate tissues for downstream RNA analysis.

Tags

Keywords: Laser-capture MicrodissectionProstate EpitheliumRNA AnalysisProstatic EpitheliumStromal CellsEpithelial CellsProstate CancerGene ExpressionRT-qPCRRNAseq

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