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En celle Gratis Assay til at studere Chromatin dekondensering i slutningen af ​​Mitose
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Biologia
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JoVE Journal Biologia
A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

En celle Gratis Assay til at studere Chromatin dekondensering i slutningen af ​​Mitose

DOI:
10.3791/53407-v

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11:04 min

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December 19, 2015

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, , ,
1Friedrich Miescher Laboratory,Max Planck Society, 2Nencki Institute of Experimental Biology,Polish Academy of Sciences

Capitoli

  • 00:05Titolo
  • 00:48Protocol for Interphasic Xenopus Laevis Egg Extract
  • 05:38Protocol for In Vitro Reconstitution of Chromatin Decondensation
  • 08:56Results: Chromatin Decondensation is ATP and GTP Dependent
  • 09:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.

Tags

Keywords: Cell-free AssayChromatin DecondensationMitosisMitotic ExitNuclear EnvelopeX. Laevis Egg ExtractHeLa CellsMolecular EventsChromatin CondensationChromosome Structure

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