Rivista
/
Immunologia e infezioni
/
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

DOI:
10.3791/53408-v

•

12:16 min

•

December 17, 2015

•

,
1Philips Institute for Oral Health Research,Virginia Commonwealth University, 2Department of Microbiology and Immunology,Virginia Commonwealth University, 3Department of Biochemistry,Virginia Commonwealth University

Capitoli

  • 00:05Titolo
  • 01:15Anaerobic Bacteria Infection Assay
  • 05:07Survival of Internalized Bacteria
  • 06:15Fluorescence Microscopy of Internalized Bacteria
  • 09:15Results: Infection of HUVECs with Porphyromonas Gingivalis
  • 10:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

This article presents two protocols: one to measure anaerobic bacteria that can successfully invade and survive within the host, and the other to visualize anaerobic bacteria interacting with host cells. This study can be applied to any cultivable anaerobe and any eukaryotic cell type.

Tags

Keywords: Porphyromonas GingivalisAnaerobic BacteriaHost Cell InvasionAntibiotic Protection AssayFluorescent MicroscopyBCECF-AMTRITC PhalloidinDAPIZ-stack Imaging

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/53408?article_type=v

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
check_url/it/53408?article_type=v

Introducing Shear Stress in the Study of Bacterial Adhesion
check_url/it/53408?article_type=v

Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
check_url/it/53408?article_type=v

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
check_url/it/53408?article_type=v

Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
check_url/it/53408?article_type=v

Studying Microbial Communities In Vivo: A Model of Host-mediated Interaction Between Candida Albicans and Pseudomonas Aeruginosa in the Airways
check_url/it/53408?article_type=v

Biomimetic Materials to Characterize Bacteria-host Interactions
check_url/it/53408?article_type=v

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
check_url/it/53408?article_type=v

A Murine Model of Group B Streptococcus Vaginal Colonization
check_url/it/53408?article_type=v

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Read Article