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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔

DOI:
10.3791/53421-v

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10:02 min

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December 14, 2015

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, ,
1MRC Centre of Developmental Neurobiology,King’s College London, 2School of Biological and Chemical Sciences,Queen Mary, University of London

Capitoli

  • 00:05Titolo
  • 00:55Dissection of E14 Cerebellum
  • 03:00Slice Culture of E14 Cerebellum
  • 04:21Electroporation of Cerebellum Slices
  • 07:08Preparing Cerebellar Slices for Imaging
  • 07:58Results: Cerebellar Tissue Maintains Integrity and Proliferation in Culture
  • 09:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

小脑外部颗粒层是最大的过境扩增在显影脑的部位。在这里，我们提出了一个协议，以目标基因改造这个层扩散采用体外电击和小脑切片文化从胚胎第14天鸡胚的高峰。

Tags

Cerebellar SlicesGranule Cell PrecursorsEx Vivo CultureElectroporationCerebellar External Granule LayerNeuronal ProliferationDifferentiationEvo-devoMedulloblastomaGranule Neuron DevelopmentCerebellum DevelopmentEvolutionDisease

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