È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:00Peptide Design and Synthesis for Raising Monoclonal Antibodies
- 03:06Preparation of Colloidal Gold-labeled Monoclonal Antibody
- 05:13Construction of Immune Strip
- 06:53Cross-reactivity Test
- 08:07Results: Cetacean Myoglobin Is Specifically Detected by the Immune Colloidal Gold Test Strips
- 10:16Conclusion
This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.
