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Bioengineering

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JoVE Journal Bioengineering
Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
 

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Article DOI: 10.3791/53459-v • 13:16 min • December 22nd, 2015
December 22nd, 2015 •
1, 2,3,4, 2,3, 1, 4, 1,2,3,4, 1,2,3,4, 1,2,3
1Skeletal Biotech Laboratory, The Hebrew University–Hadassah Faculty of Dental Medicine, 2Department of Surgery, Cedars-Sinai Medical Center, 3Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, 4Biomedical Imaging Research Institute, Cedars-Sinai Medical Center

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Automatic Translation

Implantation of autologous and allogeneic bone grafts constitute accepted approaches to treat major craniofacial bone loss. Yet the effect of graft composition on the interplay among neovascularization, cell differentiation and bone formation is unclear. We present a multimodal imaging protocol aimed to elucidate the angiogenesis-osteogenesis interdependence at the graft proximity.

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Keywords: Computed Tomography Optical Imaging Osteogenesis-angiogenesis Coupling Bone Autografts Bone Allografts Vascularization Micro-CT Fluorescence Imaging Bioluminescence Imaging Bone Regeneration Stem Cell Recruitment Arteriole Formation Bone Mineralization
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