/
/
Приготовление Thermoresponsive наноструктурированных поверхностей для тканевой инженерии
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:15Synthesis of Polystyrene-block-poly(N-isopropylacrylamide) by Two-step Reversible Addition-fragmentation Chain Transfer (RAFT) Radical Polymerization
- 02:42Preparation of Silanized Hydrophobic Modified Glass Substrates
- 03:49Preparation of Langmuir Films and Film-transferred Surface
- 06:59Culturing Cells and Optimizing Cell Adhesion and Detachment on the Langmuir Film Transferred Surface
- 09:32Results: Fabrication of Langmuir Film-transferred Surface and Evaluation of Cell Adhesion, Detachment, and Cell Sheet Recovery
- 11:20Conclusion
Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.
