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Spårning och kvantifiera utvecklingsprocesser i<em> C. elegans</em> Använda Open-source-verktyg
JoVE Journal
Biologia
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JoVE Journal Biologia
Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools
DOI:

10:41 min

December 16, 2015

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Capitoli

  • 00:05Titolo
  • 00:523D Cell Shape Model Reconstruction with IMOD
  • 03:08Fluorescently-labeled Structure Tracking with Endrov
  • 05:33Cortical Actomyosin Flow Analysis with PIVIab
  • 08:04Results: Representative C. elegans Developmental Tracking Analyses
  • 09:39Conclusion

Summary

Traduzione automatica

Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.

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