Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)

JoVE Journal
Biologia

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JoVE Journal Biologia

DOI:

10.3791/53510-v

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10:05 min

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January 20, 2016

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Graham S. Erwin, Matthew P. Grieshop, Devesh Bhimsaria², Asuka Eguchi, José A. Rodríguez-Martínez, Aseem Z. Ansari⁴

¹Department of Biochemistry,University of Wisconsin–Madison, ²Department of Electrical and Computer Engineering,University of Wisconsin–Madison, ³Graduate Program in Cellular and Molecular Biology,University of Wisconsin–Madison, ⁴The Genome Center,University of Wisconsin–Madison

Capitoli

00:05Titolo
00:48Crosslinking in Live Cells
03:19Isolation of Chromatin
05:14Capture of Ligand-DNA Crosslinks and Isolation of Affinity-purified DNA
07:52Results: COSMIC Indentifies Genome-wide Binding Events of Polyamides
09:21Conclusion

Summary

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Identifying the direct targets of genome-targeting molecules remains a major challenge. To understand how DNA-binding molecules engage the genome, we developed a method that relies on crosslinking of small molecules to isolate chromatin (COSMIC).

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)

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