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Clonagem Funcional Utilizando um<em> Xenopus</em> Oocyte Sistema de Expressão
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Capitoli
- 00:05Titolo
- 00:53Preparation of Oocytes
- 02:21Injection of Library Transcripts
- 04:08Preparing the Animal Cap Asssay
- 05:48Generation and Culture of Recombinants
- 07:35Results: Identification of a Gene Product that Elicits a Lens-Inductive Response in Animal Cap Ectoderm
- 09:11Conclusion
We describe a Xenopus oocyte and animal cap system for the expression cloning of genes capable of inducing a response in competent ectoderm, and discuss techniques for the subsequent analysis of such genes. This system is useful in the functional identification of a wide range of gene products.
