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全山解剖和成年小鼠耳蜗的免疫
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Neuroscienze
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JoVE Journal Neuroscienze
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
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全山解剖和成年小鼠耳蜗的免疫

DOI:
10.3791/53561-v

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12:02 min

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January 01, 2016

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1Department of Surgery, Division of Otolaryngology,Southern Illinois University, School of Medicine, 2Department of Pharmacology,Southern Illinois University, School of Medicine

Capitoli

  • 00:05Titolo
  • 00:59Preparation of Temporal Bones
  • 02:14Whole Mount Dissection of the Cochlea
  • 05:41Immunostaining with Fluorescently Tagged Antibodies
  • 07:30Mounting Stained Cochlear Turns
  • 08:59Results: Examples of Optimal and Suboptimal Dissection and Immunostaining
  • 10:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提出了一个方法，以分离科尔蒂的成年器官三个完整耳蜗匝（先端，中间，和碱）。我们还演示了一个程序，用荧光标记的抗体免疫染色。连同这些技术允许毛细胞，支持细胞，以及其他细胞类型中的耳蜗研究发现。

Tags

Keywords: CochleaWhole Mount DissectionImmunofluorescenceOrgan Of CortiTemporal BoneOtic CapsuleSpiral LigamentDecalcificationForcepsVannas-Tubingen ScissorsConfocal MicroscopyZ-axis Imaging

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