/

Biologia dello sviluppo

/

从鼠耳和尾组织生成主成纤维细胞培养

JoVE Journal
Biologia dello sviluppo

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia dello sviluppo

Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

Please note that all translations are automatically generated. Click here for the English version.

从鼠耳和尾组织生成主成纤维细胞培养

DOI:

10.3791/53565-v

•

06:35 min

•

January 10, 2016

•

Muznah Khan, Stephan Gasser²

¹Immunology Program, Department of Microbiology,Yong Loo Lin School of Medicine, National University of Singapore, ²NUS Graduate School for Integrative Sciences and Engineering,National University of Singapore

Capitoli

00:05Titolo
00:42Extraction of Fibroblasts from Ear and Tail Tissues
03:20Cell Culture and Sub-Culturing of Fibroblasts
05:05Results: Fibroblast Culture Hardiness and Purity
06:03Conclusion

Summary

Traduzione automatica

我们描述用于产生从耳朵和小鼠的尾部的成纤维细胞初级一个简单而快速的实验程序。该过程不需要特殊的动物训练和可用于成纤维细胞培养的产生从存储在RT至10天耳朵。

Tags

Primary Fibroblast Mouse Ear Mouse Tail Cell Isolation Collagenase Digestion Cell Strainer Cell Suspension Cell Washing

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

在成年小鼠V-SVZ细胞的神经分析的稳定，高效的基因修饰干细胞自主和非自主的影响

小鼠皮肤成纤维隔离用流式细胞仪

从人类冷冻胃肠标本分离CD 90+成纤维细胞/肌成纤维细胞

使用主神经球文化研究纤毛

成人皮肤角质形成细胞的生成与培养

成人小鼠成纤维细胞对红细胞生成子细胞的直接线重编程

小鼠颅面组织和未切除骨骼的组织制备及免疫染色

小鼠骨骼肌肉外泄原发性肌基器的分离和分化

使用纸屑小鼠研究矿化组织的克隆遗传追踪

微挥和荧光素-提拉美化物，用于小鼠肾上腺的多重免疫染色 – 使用来自同一宿主物种的未结合原抗体

Read Article