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利用双光子显微镜的艾滋病毒引起的神经炎症的小鼠模型脑血管结构的量化

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation

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利用双光子显微镜的艾滋病毒引起的神经炎症的小鼠模型脑血管结构的量化

DOI:

10.3791/53582-v

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10:04 min

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January 12, 2016

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Christopher Nishimura, Oksana Polesskaya, Stephen Dewhurst, Jharon N. Silva

¹Department of Microbiology and Immunology,University of Rochester Medical Center, ²Department of Human Genetics,University of Chicago

Capitoli

00:05Titolo
00:50Preparation of the Thin-skull Cranial Window
02:41Monitoring of Physiological Parameters
04:02Injection of the Fluorescent Dye
04:54In Vivo Two-Photon Imaging
05:42Ex Vivo Two Photon Imaging
08:04Results: Generation of Skeletonized Capillary Networks
09:17Conclusion

Summary

Traduzione automatica

This paper describes a method by which the vascular architecture in the brain can be quantified using in vivo and ex vivo two-photon microscopy.

Tags

Keywords: Two-photon Microscopy Cerebral Vascular Architecture HIV-induced Neuroinflammation Mouse Model Capillary Morphology Neurocognitive Disorders Alzheimer’s Disease Anesthesia Skull Thinning Head Plate Surgical Procedure

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