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Capitoli
- 00:05Titolo
- 00:37Editing Pluripotent Stem Cells
- 05:00Picking Transfected Colonies
- 07:54Results: Comparison of EGFP-positive and Targeted TALEN, ZFN and CRISPR/Cas9 Human Stem Cell Colonies
- 09:00Conclusion
Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.
