Rivista
/
Biologia
/
Meiotiske Spindel Vurdering i museoocytter af siRNA-medieret Silencing
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotiske Spindel Vurdering i museoocytter af siRNA-medieret Silencing

DOI:
10.3791/53586-v

•

09:16 min

•

October 11, 2015

•

,
1Department of Physiology and Pharmacology,College of Veterinary Medicine, University of Georgia

Capitoli

  • 00:05Titolo
  • 00:54Collecting Mouse Oocytes
  • 02:08Oocyte Microinjection
  • 04:42Culturing and Staining the Injected Oocytes
  • 07:52Results: Disruption of Pericentrin Expression in Oocytes during Meiotic Division
  • 08:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her præsenterer vi en protokol for specifik siRNA-medieret mRNA udtømning efterfulgt af immunofluorescensanalyse at evaluere meiotisk spindel samling og organisation i museoocytter. Denne protokol er velegnet til in vitro-udtømning af udskrifter og funktionel vurdering af forskellige spindel og / eller MTOC-associerede faktorer i oocytter.

Tags

Meiotic SpindleSiRNAChromosome SegregationAneuploidyMicrotubule Spindle ApparatusMTOCMouse OocytesImmunofluorescence Analysis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Nuclear Transfer into Mouse Oocytes

Retrieval of Mouse Oocytes

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

Flow Cytometry Purification of Mouse Meiotic Cells

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Langsigtet undertrykkelse af Intersectin-1s i muselunger ved Gentagen leveringen af ​​en specifik siRNA via kationiske liposomer. Evaluering af Knockdown Effects ved elektronmikroskopi

In Vivo 4-Dimensional sporing af hæmatopoietisk stamcelletransplantation og stamcelle i Voksen Mouse calvarie knoglemarv

Read Article