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Capitoli
- 00:05Titolo
- 00:56Assay Mixture Preparation
- 02:24Droplet Generation and PCR Plate Setup
- 05:17Thermal Cycling and Droplet Reading
- 08:01Data Analysis and Reporting
- 09:46Results: Duplex Digital PCR is a Robust Assay for Simultaneous Quantification of General and Human-associated Fecal Contamination
- 11:18Conclusion
Tags
Duplex Digital PCR AssayQuantificationEnterococcus Spp.Human Fecal-associated HF183 MarkerWatersFecal ContaminationRecreational Water Quality MonitoringAssay AdvantagesQPCRStandard CurvesBiasVariabilityProcedure DemonstrationStock ConcentrationsPrimersMolecular Grade WaterProbesTEPH 8 BufferMaster Mix PreparationPipetting TechniqueHomogenous SolutionAssay MixtureDroplet Gen
